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human foreskin fibroblasts hf crl  (ATCC)


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    Structured Review

    ATCC human foreskin fibroblasts hf crl
    Human Foreskin Fibroblasts Hf Crl, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 481 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+foreskin+fibroblasts+bj+5ta/BJ-5ta/pm42173947-418-0-9
    Average 96 stars, based on 481 article reviews
    human foreskin fibroblasts hf crl - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Multiple Displacement Amplification:

    Article Title: Inhibition of Carbonic Anhydrase IX Suppresses Breast Cancer Cell Motility at the Single-Cell Level
    Article Snippet: .. Human breast adenocarcinoma cell lines MDA-MB-231 and MCF-7, human foreskin fibroblasts BJ-5ta were obtained from the American Type Culture Collection (Manassas, VA, USA) and were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, Grand Island, NY, USA), supplemented with 10% Fetal Bovine Serum (FBS) (Gibco, Grand Island, NY, USA), 1% Penicillin-Streptomycin (10,000 U/mL) (Gibco, Grand Island, NY, USA) at 37 °C in a humidified atmosphere with 5% CO 2 and 21% O 2 until passage number of 20. .. MDA-MB-231, MCF-7, and BJ-5ta cells were grown on glass coverslips under normoxia- and hypoxia-mimicking conditions (by adding 240 μM CoCl 2 (Merck, Kenilworth, NJ, USA) as a hypoxia mimetic agent into the cell growing medium) for 48 h and then fixed with ice-cold methanol for 20 min. Then methanol was aspirated, and cells were incubated with an incubation buffer (PBS-Gibco, Grand Island, NY, USA) containing 2% bovine serum albumin (Sigma-Aldrich, St. Louis, MO, USA) and 2% FBS.

    Cell Culture:

    Article Title: Inhibition of Carbonic Anhydrase IX Suppresses Breast Cancer Cell Motility at the Single-Cell Level
    Article Snippet: .. Human breast adenocarcinoma cell lines MDA-MB-231 and MCF-7, human foreskin fibroblasts BJ-5ta were obtained from the American Type Culture Collection (Manassas, VA, USA) and were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, Grand Island, NY, USA), supplemented with 10% Fetal Bovine Serum (FBS) (Gibco, Grand Island, NY, USA), 1% Penicillin-Streptomycin (10,000 U/mL) (Gibco, Grand Island, NY, USA) at 37 °C in a humidified atmosphere with 5% CO 2 and 21% O 2 until passage number of 20. .. MDA-MB-231, MCF-7, and BJ-5ta cells were grown on glass coverslips under normoxia- and hypoxia-mimicking conditions (by adding 240 μM CoCl 2 (Merck, Kenilworth, NJ, USA) as a hypoxia mimetic agent into the cell growing medium) for 48 h and then fixed with ice-cold methanol for 20 min. Then methanol was aspirated, and cells were incubated with an incubation buffer (PBS-Gibco, Grand Island, NY, USA) containing 2% bovine serum albumin (Sigma-Aldrich, St. Louis, MO, USA) and 2% FBS.

    Modification:

    Article Title: Inhibition of Carbonic Anhydrase IX Suppresses Breast Cancer Cell Motility at the Single-Cell Level
    Article Snippet: .. Human breast adenocarcinoma cell lines MDA-MB-231 and MCF-7, human foreskin fibroblasts BJ-5ta were obtained from the American Type Culture Collection (Manassas, VA, USA) and were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, Grand Island, NY, USA), supplemented with 10% Fetal Bovine Serum (FBS) (Gibco, Grand Island, NY, USA), 1% Penicillin-Streptomycin (10,000 U/mL) (Gibco, Grand Island, NY, USA) at 37 °C in a humidified atmosphere with 5% CO 2 and 21% O 2 until passage number of 20. .. MDA-MB-231, MCF-7, and BJ-5ta cells were grown on glass coverslips under normoxia- and hypoxia-mimicking conditions (by adding 240 μM CoCl 2 (Merck, Kenilworth, NJ, USA) as a hypoxia mimetic agent into the cell growing medium) for 48 h and then fixed with ice-cold methanol for 20 min. Then methanol was aspirated, and cells were incubated with an incubation buffer (PBS-Gibco, Grand Island, NY, USA) containing 2% bovine serum albumin (Sigma-Aldrich, St. Louis, MO, USA) and 2% FBS.

    other:

    Article Title: Synthesis and Anticancer Evaluation of PCNA Inhibitor AOH1996 Analogs in Cancer Cell Cultures
    Article Snippet: The human breast cancer cell line MCF-7 (ATCC HTB-22) and the human glioblastoma cell line U87 (ATCC HTB-14) were originally obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and kindly provided by Prof. Daumantas Matulis (Vilnius University, Vilnius, Lithuania).



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